Evaluation of the interaction between SARS-CoV-2 spike glycoproteins and the molecularly imprinted polypyrrole
نویسندگان
چکیده
The SARS-CoV-2 spike glycoprotein (SARS-CoV-2-S) was used as a template molecule and polypyrrole (Ppy) applied an electro-generated conducting polymer, which acting matrix for the formation of molecular imprints. Two types Ppy-layers: molecularly imprinted (MIP-Ppy) non-imprinted (NIP-Ppy) were electrochemically deposited on working platinum electrode. performance electrodes modified by MIP-Ppy NIP-Ppy layers evaluated pulsed amperometric detection (PAD). During assessment measurement results registered PAD, integrated Cottrell equation (Anson plot) to calculate amount charge passed through layers. interaction between glycoproteins assessed Anson plot based calculations. This reveals that SARS-CoV-2-S are interacting with more strongly than NIP-Ppy.
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ژورنال
عنوان ژورنال: Talanta
سال: 2023
ISSN: ['0039-9140', '1873-3573']
DOI: https://doi.org/10.1016/j.talanta.2022.123981